estimation of the cultured cells’ volume and surface area: application of stereological methods on vero cells infected by rubella virus

نویسندگان

ali noorafshan histomorphometry and stereology research center, shiraz university of medical sciences, shiraz, iran

mohammad motamedifar hiv/aids research centre (sharc), department of bacteriology and virology, school of medicine, shiraz university of medical sciences, shiraz, iran

saied karbalay-doust histomorphometry and stereology research center, shiraz university of medical sciences, shiraz, iran

چکیده

background: morphological changes of the cells infected with rubella virus cannot be observed easily. estimation of the size of the cultured cells can be a valuable parameter in this condition. this study was conducted to find answers to the following questions: how much time after infection with rubella virus, the volume and surface area of the vero cells and their nuclei get started to change? how is it possible to apply stereological methods to estimate the volume and surface area of the cultured cells using the invariator, nucleator, and surfactor techniques? methods: the cultured vero cells were infected with rubella virus. the cells of the control and experimental groups were harvested at 2, 4, 8, 24, and 48 hours following the incubation period. the cells were processed and embedded in paraffin. invariator, nucleator, and surfactor were applied to estimate the size of the vero cells and their nuclei. results: the cell volume was decreased by 15-24%, 48 hours after the infection in comparison to the non-infected cells. besides, the cell surface area was decreased by 13%, 48 hours after the infection. however, no changes were detected in the nuclei. the values of the standard deviation and coefficient of variation of the cells, estimated by invariator, were lower compared to those measured by the nucleator or surfactor. conclusion: in this study, the volume and surface area of the vero cells were reduced by rubella virus 48 hours after infection. invariator is a more precise method compared to nucleator or surfactor.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Estimation of the Cultured Cells’ Volume and Surface Area: Application of Stereological Methods on Vero Cells Infected by Rubella Virus

Background: Morphological changes of the cells infected with rubella virus cannot be observed easily. Estimation of the size of the cultured cells can be a valuable parameter in this condition. This study was conducted to find answers to the following questions:How much time after infection with rubella virus, the volume and surface area of the Vero cells and their nuclei get started to change?...

متن کامل

Estimation of the Cultured Cells’ Volume and Surface Area: Application of Stereological Methods on Vero Cells Infected by Rubella Virus

BACKGROUND Morphological changes of the cells infected with rubella virus cannot be observed easily. Estimation of the size of the cultured cells can be a valuable parameter in this condition. This study was conducted to find answers to the following questions: How much time after infection with rubella virus, the volume and surface area of the Vero cells and their nuclei get started to change?...

متن کامل

on the comparison of keyword and semantic-context methods of learning new vocabulary meaning

the rationale behind the present study is that particular learning strategies produce more effective results when applied together. the present study tried to investigate the efficiency of the semantic-context strategy alone with a technique called, keyword method. to clarify the point, the current study seeked to find answer to the following question: are the keyword and semantic-context metho...

15 صفحه اول

Pathway of rubella virus infectious entry into Vero cells.

The mechanism and the kinetics of rubella virus (RV) penetration into Vero cells were studied. By using pronase or acid treatment to inactivate virus which had adsorbed to cell membrane but had not been internalized, it was found that a period of 7 h was required in order for all of the adsorbed virus to enter the host cells. Lysosomotropic agents (monensin, methylamine, ammonium chloride and c...

متن کامل

the effects of error correction methods on pronunciation accuracy

هدف از انجام این تحقیق مشخص کردن موثرترین متد اصلاح خطا بر روی دقت آهنگ و تاکید تلفظ کلمه در زبان انگلیسی بود. این تحقیق با پیاده کردن چهار متد ارائه اصلاح خطا در چهار گروه، سه گروه آزمایشی و یک گروه تحت کنترل، انجام شد که گروه های فوق الذکر شامل دانشجویان سطح بالای متوسط کتاب اول passages بودند. گروه اول شامل 15، دوم 14، سوم 15 و آخرین 16 دانشجو بودند. دوره مربوطه به مدت 10 هفته ادامه یافت و د...

15 صفحه اول

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
iranian journal of medical sciences

جلد ۴۱، شماره ۱، صفحات ۳۷-۰

کلمات کلیدی

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023